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重組小鼠白介素-3(rmIL-3)

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CYT-371重組小鼠白介素-3(rmIL-3) 2μg/10μg/1mg Prospec

 

 

Background :
 Interleukin-3 (IL-3) is a cytokine produced by activated T-cells and mast cells that is active on a broad range of hematopoietic cells and in the nervous system and appears to be important in several chronic inflammatory diseases

 
Description :
 Recombinant murine IL-3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton.
Recombinant Murine IL-3 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 Interleukin-3 is lyophilized from a concentrated (1mg/ml) solution in water containing no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized IL-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Interleukin-3 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 ProSpec’s Murine IL-3 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 2 x 107 IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant IL-3.

 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Murine IL-3 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 


 佰易聚生物商城提供美國光譜醫學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!

 

 

 

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