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重組大鼠白介素-1β(rrIL-1β)

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更新時間:2025-05-02 14:56:39瀏覽次數:1220次

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CYT-394重組大鼠白介素-1β(rrIL-1β) 2μg/10μg/1mg Prospec

 







 

Background:
 A soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. IL-1 consists of two distinct forms, IL-1 alpha and IL-1 beta which perform the same functions but are distinct proteins.
The synthesis of IL-1 can be induced by other cytokines including TNF-alpha , IFN-alpha , IFN-beta and IFN-g and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 alpha and TNF-alpha induce the synthesis of IL-1beta . In pheochromocytoma cells, NGF induces the synthesis of IL-1.

 
Description :
 Recombinant Rat Interleukin-1 beta produced in E.Coli is a non-glycosylated, Polypeptide chain containing 153 amino acids and having a molecular mass of 17415.82 Dalton.
The Recombinant Rat IL-1b is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=6.5.

 
Solubility:
 It is recommended to reconstitute the lyophilized Rat Interleukin-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized Rat IL-1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-1 beta should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

 
Amino-Acid Sequence :
 The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Pro-Ile-Arg.

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 The ED50 as determined by the dose-dependant stimulation of murine D10S cells is < 0.1 ng/ml, corresponding to a Specific Activity of 1 x 107 IU/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant Rat Interleukin-1 beta.

 
Protein content:
 Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Rat IL-1b as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

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