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CYT-350

重組小鼠白介素-6（rmIL-6）

2μg/10μg/1mg

Prospec

Background:
	Interleukin （IL）-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells.Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
	Description :
	Recombinant Murine IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21709 Dalton. Mouse IL-6 is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	The protein was lyophilized from a concentrated （1mg/ml） solution containg 50 mM NH4HCO3, pH 8.0.
	Solubility:
	It is recommended to reconstitute the lyophilized rmIL-6 in 100 mM acetic acid to 0.1-1.0 mg/mL to regain full activity, and can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized IL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-6 should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less then 0.02 ng/ml, corresponding to a Specific Activity of 1 x 10 ⁷IU/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of IL-6.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm. 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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