24 roundwell microplates 24個圓孔微孔板

24 roundwell microplates 24個圓孔微孔板

24個圓井微孔板（例如康寧公司生產的）CHO細胞的標準條件：•0.75-1毫升培養液•在225轉/分時的50毫米震動幅度，或在300轉/分時的25毫米震動幅度•三明治蓋CR1524a或CR1324a•夾鉗CR1800（可堆疊三個板+三明治蓋，（見下面的照片）進一步的細節：這種平板格式已經被用于CHO細胞很多年了，從CHO細胞系沒有現在那么健壯開始。在倫敦大學學院和劍橋醫學院（英國）的一個合作項目中，從康寧和我們的三明治蓋和夾鉗（Silk等人，2010年，《生物技術快報》，32:73-78）中驗證并優化了CHO細胞的培養，軌道振動產生溫和的混合（非常低的剪切應力），因此即使是脆弱的動物細胞系也可以在這些板中培養。這些板的另一個優點（與上面所述的正方形深孔微滴定板相比）是由兩個壁而不是一個壁分開的。進一步降低了交叉污染的可能性。此外，康寧公司生產的24個圓孔板在聚苯乙烯與蛋白質的結合特性方面具有不同的質量（TC處理、超低附著、未處理等）

24 roundwell microplates (e.g. from Corning)Standard conditions for CHO-cells:•0.75-1 ml culture volumes •50 mm shaking amplitude at 225 rpm, or •25 mm shaking amplitude at 300 rpm •sandwich covers CR1524a or CR1324a •clamp CR1800 (three plates + sandwich covers can be stacked, see photo below)Further details:This plate format has been used already for many years for CHO-cells, starting in the time that CHO-cell lines were less robust than that they are at present. In a collaborative project of the UCL in London and Medimmune in Cambridge (UK), the c*tion of CHO-cells was validated and optimized in these plates from Corning and our sandwich covers and clamps (Silk et al, 2010, Biotechnology Letters, 32:73–78).Because of the round well format, orbital shaking gives rise to a gentle mixing (very low shear stress), and so even the most vulnerable animal cell lines can be c*ted in these plates.Another advantage of these plates (in comparison to the square-deepwell microtiter plates as described above) is that the cultures are separated by two walls rather than one, which further reduces the chance of cross-contamination.Furthermore, the 24-roundwell plates from Corning are available in various qualities in terms of binding characteristics of the polystyrene for proteins (TC-treated, ultra-low attachment, untreated, etc.)

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