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描述

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With	Without
• High Glucose	• Sodium Pyruvate
• L-glutamine	• HEPES
• Phenol Red	• Sodium Bicarbonate

The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (52100-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

規(guī)格

Glutamine:	L-Glutamine
Phenol Red Indicator:	Phenol Red
Form:	Powder
Glucose:	High Glucose
Serum Supplementation:	Standard Serum Supplementation
HEPES Buffer:	No HEPES
Sodium Pyruvate Additive:	No Sodium Pyruvate
Product Line:	Gibco®
Product Size:	10 x 1 L
Sodium Bicarbonate Buffer:	No Sodium Bicarbonate
Shipping Condition:	Room Temperature

內(nèi)容及儲存

儲存條件：2-8° C
運(yùn)輸條件：環(huán)境溫度
保存期：自生產(chǎn)日期起36個月