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| CBP60297 | |
| I. General information | |
| Synonyms: | CHO |
| Background: | A cell line originally derived from Chinese Hamster Ovary cells by Puck in 1957. The cells have an absolute requirement for L-proline |
| Species: | Hamster |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Gender: | N/A |
| Morphology: | Epithelial |
| Growth Mode: | Adherent |
| Doubling Time: | N/A |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: | F12K+10%FBS We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | 90%FBS+10%DMSO |
| Karyotype: | Hypodiploid, modal no. 20 |
| Subculture Routine: | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. |
| Comments: | For more information, please contact Cobioer (4008-750-250). |
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