BCR-ABL1 Y253H/T315I/BaF3
| CBP73263 | |
| I. Introduction | |
Cell Line Name： | BCR-ABL1 [Y253H/T315I]/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
Presence of a BCR-ABL1 fusion gene is necessary for the pathogenesis of CML. In up to 95% of cases, a t(9;22) (q34;q11) translocation results in the BCR-ABL1 fusion gene (Faderl et al. 1999). This translocation results in the Philadephia chromosome. In rare CML cases lacking the traditional t(9;22) translocation, other translocations result in the creation of the BCR-ABL1 fusion gene, which sometimes involve multiple chromosomes. ABL1 is a tyrosine kinase, and, in normal cells, it plays a role in cellular differentiation and regulation of the cell cycle. The BCR-ABL1 fusion gene creates a constitutively active tyrosine kinase, which leads to uncontrolled proliferation. | |
| III. Representative Data | |
1. WB of BCR-ABL1 [Y253H/T315I]/BaF3
| |
2.Sanger of BCR-ABL1 [Y253H/T315I]/BaF3
Figure 2. BCR-ABL1 Y253H
Figure 3. BCR-ABL1 T315I
Figure 4. BCR ABL1 Breakpoint | |
3. Anti-proliferation assay | |
| |
Figure 5. CTG Proliferation Assay of BaF3 BCR-ABL1 T315I Y253H (C2). | |
化工儀器網