CBP73286 | |
| I. Introduction | |
Cell Line Name： | c-Kit V559A/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT V559A lies within the juxtamembrane domain (exon 11) of the Kit protein (PMID: 15837988). V559A results in constitutive phosphorylation of Kit and increased Mapk and Akt activation in patient samples and cultured cells (PMID: 15837988, PMID: 19047099). | |
| III. Representative Data | |
1.Sanger of c-Kit V559A/BaF3
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2. Anti-proliferation assay
Figure 2. CTG Proliferation Assay of BaF3 c-KIT V559A (C2). | |
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