| CBP73214 | |
| I. Introduction | |
Cell Line Name： | CCDC6-RET WT/BaF3 |
Host Cell: | BA/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+1ug/ml puromycin |
Mycoplasma Status: | Negative |
| II.Background | |
CCDC6, coiled-coil domain containing 6, has been shown to play a role in the DNA damaging signaling pathway acting as a substrate of ATM (PMID: 17420723). CCDC6 fusions with RET (PMID: 23154560, PMID: 26078337) and BRAF (PMID: 26314551) have been observed in multiple cancer types. | |
| III. Representative Data | |
1. WB of CCDC6-RET WT/BaF3WT
| |
2. Sanger of CCDC6-RET WT/BaF3
Figure 2. Breakpoint of CCDC6-RET WT/BaF3 | |
2. Anti-proliferation assay | |
Figure 3. CTG assay of for CCDC6-RET WT/BaF3 Stable Cell Line,the Gi50 was 137.3nM. | |
化工儀器網