| CBP73117 | |
| I. Introduction | |
Cell Line Name： | TEL(ETV6)-JAK2/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | This kit is useful for screening for small molecules displacing ATP from the JH2 domain of JAK2. |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS+2ug/ml puromycin |
Mycoplasma Status: | Negative |
| II.Background | |
JAK2, Janus kinase 2, is a non-receptor protein tyrosine kinase that binds cytokine receptors to activate the interferon-alpha/beta/gamma pathway and is a member of the JAK/STAT signaling pathway (PMID: 15575979, PMID: 30092288). JAK2 mutations are observed in a variety of tumor types, including myeloproliferative neoplasms (PMID: 30074114), lymphoma (PMID: 29984752), leukemia (PMID: 29979407), and cervical cancer (PMID: 30003068). | |
| III. Representative Data | |
1.WB of BA/F3/TEL(ETV6)-JAK2
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2. Anti-proliferation assay | |
Figure 2. CTG Proliferation Assay of BaF3 TEL-JAK2 (C1). | |
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