| CBP30105L | |
| I. Introduction | |
| Host Cell: | MCF7(ER positive) |
| Expressed gene: | Luciferase |
| Stability: | 32 passages(in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)+ 10 µg/ml human insulin+ 1ug/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen |
| II. Description of Host Cell Line | |
| Organism: | Homo sapiens, human |
| Tissue: | Mammary gland/breast; derived from metastatic site: pleural effusion |
| Disease: | Adenocarcinoma |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| III. Representative Data | |
| |
Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System. | |
| IV. Stablity Test | |
 | |
Figure 2. Stablity test results. MCF7-Luc cells showed at least 32 passage stability with similar luminescence unit. | |
化工儀器網