| CBP73335 | |
| I. Introduction | |
Cell Line Name： | PDGFRA D842V-T674I/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
PDGFRA D842V lies within the protein kinase domain of the Pdgfra protein (UniProt.org). D842V results in ligand-independent phosphorylation of Pdgfra, activation of downstream signaling, and is transforming in culture (PMID: 22745105, PMID: 23752188, PMID: 29533785, PMID: 30253204) and confers resistance to PDGFRA inhibitors (PMID: 30389923, PMID: 18955458). | |
| III. Representative Data | |
1.Sanger of PDGFRA D842V-T674I/BaF3
Figure 1. PDGFRA D842V-T674I/BaF3 T674I
Figure 2. PDGFRA D842V-T674I/BaF3 D842V | |
2. Anti-proliferation assay | |
Figure 3. CTG Proliferation Assay of BaF3 PDGFRA D842V/T674I Cells (C4). | |
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