| I. Background | |
趨化因子是一類炎性細胞因子，具有調節細胞遷移、粘附、吞噬作用、細胞因子分泌、 增殖和細胞凋亡的能力。越來越多的證據表明，趨化因子及其受體在穩態和炎癥條件下在 調節樹突狀細胞遷移以及穩態和炎癥功能方面發揮著重要作用。 | |
| II.Description | |
cAMP 在 G 蛋白偶聯受體（GPCR）信號轉導中是一種關鍵的第二信使。當配體結合 到 GPCR，構象發生變化，激活受體接著激活 G 蛋白，Gi 的激活導致腺苷酸環化酶調控 cAMP 的下調。CXCR2 CHO 藥靶模型很好的模擬了體內 CXCR2 的信號轉導過程，原理見 下圖所示。
Figure 1. CXCR2 CHO 細胞模型原理圖 | |
| III. Introduction | |
| Host Cell: | CHO |
| Stability: | 20 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5 μg/ml Puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for CXCR2 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 3. HTRF cAMP Assay with CXCR2 CHO(C7). | |
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