CBP73234 | |
| I. Introduction | |
Cell Line Name： | c-Kit V559D/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
KIT V559D lies within the juxtamembrane domain (exon 11) of the Kit protein (PMID: 9438854). V559D confers a gain of function on Kit, as indicated by constitutive Kit phosphorylation, and is transforming in cell culture (PMID: 9438854, PMID: 21689725). | |
| III. Representative Data | |
1.WB of c-Kit V559D/BaF3
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2.Sanger of c-Kit V559D/BaF3
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3. Anti-proliferation assay
Figure 2. CTG Proliferation Assay of BaF3 c-KIT V559D (C5). | |
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