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pH值梯度緩沖溶液戴安離子色譜耗材離子色譜柱美國戴安Dionex

MAb charge variant separation using a linear pH gradient.
Column: MAbPac SCX-10,10 μm, 4 × 250 mm column.
(a) Separation by pH gradient, 0% B (pH 5.6) to 100% B (pH 10.2);
(b) Separation by pH gradient, 0% B (pH 5.6) to 50% B (pH 7.9);
(c) Separation by pH gradient, 25% B (pH 6.75) to 50% B (pH 7.9).

Thermo Scientific pH buffer concentrates used in the pH gradient platform can be purchased individually or as a pair, in quantities of 125 mL or 250 mL. For added convenience, the 125 mL buffers can also be bundled with columns in a number of specifically preconfigured kits.

• The CX-1 pH gradient starter kit contains 125 mL each of buffers A and B, plus a MAbPac SCX-10, 10 μm, 4 × 250 mm column.
• The CX-1 pH gradient high throughput kit contains 125 mL each of buffers A and B, plus a MAbPac SCX-10, 5 μm, 4 × 50 mm column.
• The CX-1 pH gradient high resolution kit contains 125 mL each of buffers A and B, plus a MAbPac SCX-10, 5 μm, 4 × 250 mm column.

For the ultimate flexibility, the preconfigured kits are also available as platforms, including the pH Designer Software. The options are listed in the table below:

pH值梯度緩沖溶液戴安離子色譜耗材離子色譜柱美國戴安Dionex，