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上海橋星貿易有限公司
主營產品： 進口透析袋,密理博ECL發光液,B27無血清培養基,Gibco膠原酶 |
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上海橋星貿易有限公司
主營產品： 進口透析袋,密理博ECL發光液,B27無血清培養基,Gibco膠原酶 |
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N2615 RNasin Plus Promega試劑?
原裝*，質量保證，現貨供應
規格：10000u
N2615 RNasin Plus Promega試劑? 產品描述：
組分：
RNasin® Plus RNase Inhibitor【子貨號：N261B，包裝：1 x 10,000u，，運保溫度：–20°C】
描述：
說明 |
| RNasin® Plus RNase Inhibitor (RNasin® RNA 酶抑制劑) 是一種作為可溶性蛋白在E. coli 中表達的重組哺乳動物RNase 抑制劑，可以利用離子交換和疏水層析相結合的方法純化獲得。該蛋白能夠抑制真核RNase( 如RNase A 和RNase B) 活性，與人胎盤RNase 抑制劑相似。RNasin®Plus RNase Inhibitor 經過RT-PCR 檢驗，能與AMV、M-MLV 和ImProm-II™ Reverse Transcriptases 或Taq 及Tfl DNA Polymerases 兼容。RNasin® Plus RNase Inhibitor 在TaqMan®檢測中發現也能和定量、實時RT-PCR 兼容。
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| 特點 | . 增強的抗氧化能力：由于天然氨基酸的多樣性，RNasin® Plus RNase Inhibitor 不能形成人源蛋白在氧化條件下會形成的封閉 |
原廠資料：
RNasin® Plus RNase Inhibitor is a recombinant mammalian RNase inhibitor that is expressed as a soluble protein in E. coli, allowing easy purification through a combination of ion exchange and hydrophobic interaction chromatography. The protein is capable of inhibiting eukaryotic RNases (e.g., RNase A and RNase B) similarly to human placental RNase inhibitor. RNasin® Plus RNase Inhibitor is tested in RT-PCR and is compatible with enzymes such as AMV, M-MLV and ImProm-II™ Reverse Transcriptases or Taq and Tfl DNA Polymerases. RNasin® Plus RNase Inhibitor also is tested and compatible with quantitative, real-time RT-PCR in a TaqMan® assay.
The inhibitor offers increased resistance to oxidation over the human version of the protein. Two cysteines in the human protein have been identified as especially sensitive to oxidation and react by forming a disulfide bond that can block the active site of the inhibitor. RNasin® Plus, through natural amino acid diversity, lacks the ability to form this site-blocking disulfide. In addition, the new protein has characteristics never before realized, including continued inhibition of RNases above 50°C. Heating solutions of RNasin® Plus and RNase followed by cooling does not result in the reappearance of RNase activity—even when the solution is heated above the denaturation temperature of the RNasin® Plus protein alone. This allows RNasin® Plus to protect RNA species prior to, during and after heating, even at temperatures normally used during first-strand DNA synthesis in RT-PCR. We have taken solutions up to 70°C for 15 minutes and did not see RNase reactivation.
? 相關產品列表：
| Promega 系列 | ||
| 序號 | 貨號 名稱 | 規格 |
| 1 | M1801 T4連接酶 | 100U |
| 2 | M1804 T4連接酶 | 500U |
| 3 | M1701 M-MLV | 10000U |
| 4 | M1705 M-MLV | 50000U |
| 5 | M5101 AMV | 300U |
| 6 | M5108 AMV | 1000U |
| 7 | M7741 Pfu | 100U |
| 8 | M7745 Pfu | 500U |
| 9 | C1101 Olig(dt)15 Primer | 20ug |
| 10 | M3001 Go-Taq | 100U |
| 11 | M3005 Go-Taq | 500U |
| 12 | M3008 Go-Taq | 2500U |
| 13 | U1511 dNTP | 200μl |
| 14 | U1515 dNTP | 1000μl |
| 15 | A1360 pGEM-T easy | 1.2ug |
| 16 | A3600 pGEM-T | 1.2ug |
| 17 | C1181 Random Primers | 20μg |
| 18 | N2511 Recombinant RNasin | 2500U |
| 19 | N2515 Recombinant RNasin | 10000U |
| 20 | N2611 RNasin Plus | 2500U |
| 21 | N2615 RNasin Plus | 10000U |
| 22 | N2111 RNasin | 2500U |
| 23 | N2115 RNasin | 10000U |
| 24 | M1871 TdT | 300U |
| 25 | M1875 TdT | 1500U |
| 26 | M2825 CIAP | 1000U |
| 27 | M1821 CIAP | 1000U |
| 28 | M2201 Klenow I | 150U |
| 29 | M2206 Klenow I | 500U |
| 30 | M4211 T4 DNA Polymerase | 100U |
| 31 | M4215 T4 DNA Polymerase | 500U |
| 32 | M4101 T4 Polynucleotide Kinase | 100U |
| 33 | M4103 T4 Polynucleotide Kinase | 1000U |
