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供貨周期	兩周	規格	1*96wells
貨號	ADI-930-001	應用領域	生物產業
主要用途	科研

Ultrasensitive measurement, detecting as little as 8.26 pg/ml PGE2
Validated in a wide variety of sample matrices
Reliable and reproducible results lot-after-lot and day-after-day
Fully quantitative results that surpass semi-quantitative Western blot analysis

The PGE2 high sensitivity EIA kit is a colorimetric competitive enzyme immunoassay kit with results overnight + 1 hour.

<strong>PGE2 high sensitivity ELISA kit</strong> Kit graph

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Product Details

Alternative Name:	Prostaglandin E2

Sensitivity:	8.26 pg/ml (range 7.8 - 1,000 pg/ml)

Assay Time:	Overnight + 1 hour

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of PGE2 in culture supernatants, serum, saliva, urine, and whole blood from any species. Cited sample types include plasma.

Wavelength:	405 nm

Species reactivity:	Species independent

Use/Stability:	Store all components at +4°C, except standard and conjugate at -20°C.

Shipping:	Shipped on Blue Ice

Contents:	GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background:	Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE2 pathway: S.G. Park, et al.; J. Vet. Sci. 22, e16 (2021), Application(s): ELISA on Feline adipose-tissue derived (fAT)-MSCs culture supernatant, Abstract; Full Text

N-acetylcysteine attenuates PGE 2 and ROS production stimulated by 4-META/MMA-based resin in murine osteoblastic cells: K. Nakamura, et al.; Dent. Mater. J. (2021), Abstract;

The COX-2/PGE2 pathway suppresses apical elimination of RasV12-transformed cells from epithelia: N. Sato, et al.; Commun. Biol. 18, 132 (2020), Abstract; Full Text

The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention: A. Rapacz-Leonard, et al.; Sci. Rep. 10, 2089 (2020), Abstract; Full Text

Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019), Application(s): ELISA using culture supernatants, Abstract;

Group VIA phospholipase A2 deficiency in mice chronically fed with high-fat-diet attenuates hepatic steatosis by correcting a defect of phospholipid remodeling: A.C. Otto, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 662 (2019), Application(s): ELISA using liver homogenates and mouse serum, Abstract;

iPla2β deficiency in mice fed with MCD diet does not correct the defect of phospholipid remodeling but attenuates hepatocellular injury via an inhibition of lipid uptake: X. Zhu, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1864, 677 (2019), Application(s): ELISA using liver homogenates, Abstract;

Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells: A. Roumane, et al.; BMC Cancer Biol. 19, 11 (2018), Abstract; Full Text

Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium: M.R. Rebordao, et al.; Theriogenology 113, 8 (2018), Abstract;

Human mesenchymal stromal cell sheets induce macrophages predominantly to an anti-inflammatory phenotype: P. Sukho, et al.; Stem Cells Dev. 27, 922 (2018), Abstract;

Impairment of the antifibrotic prostaglandin E2 pathway may influence neutrophil extracellular traps-induced fibrosis in the mare endometrium: M.R. Rebord?o, et al.; Domest. Anim. Endocrinol. 67, 1 (2018), Application(s): PGE2 concentration in equine endometrial tissue culture, Abstract;

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