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Product Introduction
Carboxyl-activated SepFast MAG is used to make magnetic chromatography media. It forms chemically stable amide linkages with ligands containing primary amine groups. It has a long spacer (9 atoms). After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet. Carboxyl-activated agarose can be used for the immobilization of amine containing molecules. The coupling chemistry is carbodiimide-based and is a successful and well-documented technique.
Product Properties:
Carboxyl-activated SepFast MAG 4HF is made of highly cross-linked 4% agarose containing magnetic material. It makes harsher operational conditions possible so that low pH and certain organic solvents of high concentrations can be applied. Agarose has long been used for chromatographic separations due to its excellent hydrophilic and low non-specific-binding nature. The particles have an open pore structure with excellent mass transfer properties to large protein molecules. The base matrix is activated by attaching a long hydrophilic spacer arm to the backbone. It reacts directly with primary amine groups in the presence of a carbodiimide agent. Carboxyl-activated SepFast MAG is supplied as an aqueous suspension in 20% ethanol.
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