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產品名稱:hct116/L人結腸癌耐細胞株、hct116/L人結腸癌耐細胞株、hct116/L人結腸癌耐細胞株、hct116/L人結腸癌耐細胞株;
細胞系特征 | ||
細胞株名稱:HCT116/L人結腸癌耐細胞株 種屬:人 組織來源:結腸癌 生長特性:貼壁生長 形態特征:上皮細胞 微生物及支原體檢測:陰性 安全性:所有腫瘤和病毒轉染的細胞均視為有潛在的生物危害性,必須在二級生物安全臺內操作,并請注意防護,所有廢液及接觸過此細胞的器皿需高壓滅菌后方能丟棄。 | ||
培養條件:
| 培養基:90%RPMI-1640+10%胎牛血清+2000ng/ml L 血清我們推薦用: GIBCOFBS-10099-141或HYCLONEFBS-SH30084.03。 培養條件:37.0C carbon dioxide(CO2),5% | |
傳代方法:
| 收到細胞后,在倒置鏡下(是在4X物鏡)觀察整個細胞生長情況。 (一)如果細胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內,嚴格無菌操作,打開細胞培養瓶,吸出培養液,換 10ml新鮮培養液后繼續培養。 (二)如果細胞已長滿,即可進行傳代培養。具體步驟如下: 1. 棄去培養液,用PBS(不含鈣,鎂離子)洗1-2次。 2. 加0.7-1ml消化液(0.25%Trypsin-0.53mM EDTA)于培養瓶中,用力拍打瓶壁,期間每隔 5-10s放到顯微鏡下觀察,直至50-70%的細胞脫落后,加入2ml 以上培養基中止消化。 3. 按6-8ml/瓶補加培養基,輕輕打勻后吸出一半,分到新的培養瓶中。如果沒有特別說明,收到細胞后的次傳代一般是一傳二。 注:1、觀察細胞在低倍鏡(4或5X物鏡)下進行,否則不能準確判斷細胞的傳代密度。看細胞的形態請在10X或20X物鏡下。 2、瓶中運輸培養基不能重復再用,請換用加雙抗的新培養基,細胞凍存后,培養基中可不加任何抗生素。 3、有些細胞貼壁不牢,如發現貼壁細胞有脫落,可離心吹打后接種到新瓶內。 4、收到細胞后,若發現培養瓶破損、有液溢出及細胞有污染,請及時與我們聯系。..
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凍存方法: | 凍存液:90%胎牛血清,10%DMSO 儲存:液氮儲存 |
choriomeningitis virus infection [ 10]. Autophagy activity may increase at the peak of CD8+ Teff responses, suggesting that autophagy could play an important role during the transition from Teff to T memory (Tmem) CD8+ cells [10]. Both studies also linked defects in CD8+ T cell memory formation to failure to induce metabolic switches, namely the upregulation of fatty acid oxidation, which is key to CD8+ T cell memory formation [10, 52, 84].
gure 1. Mechanism of autophagy in T cells
TCR-induced expression of IL-2 leads to activation of the IL-2 receptor (IL-2r),which leads to the activation of autophagy in T helper cells in a JAK1.3-dependent manner. The initiation of autophagy and formation of the limiting membrane and thenphagophore are driven by
two complexes: the ULK-complex—ULK/FIP200/Atg101/Atg13; and the Beclin-1/Vps34 complex (class III phosphatidylinositol-3-kinase complex (PI3KC3) containing Beclin-1,
Vps34,Vps15,Atg14 and Ambra1). Two cascades of ubiquitin-like conjugation systems
regulate the elongation and maturation of the denovo formed double-membrane vesicle to form the autophagosome, which eventually fuses with the lysosome to degrade cargo. The lipidation of LC3 to PE allows association with the membranes of the autophagosome. This allows specific substrates to be targeted to the autophagosome by interaction with LC3 via LC3-interacting motifs as well as autophagic cargo receptors with LC3-interacting regions. The specific sequestration and degradation of substrates complements in-bulk mechanisms of degradation of organelles, macromolecules (i.e. proteins, lipids, glycogen),and other
cellular components.
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