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皮膚惡性黑色素瘤細(xì)胞SK-MEL-5

種屬	人
別稱	SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel
組織來源	皮膚
疾病	皮膚黑色素瘤
傳代比例/細(xì)胞消化	1:2傳代,消化2-3分鐘
培養(yǎng)基配置	MEM培養(yǎng)基；10%胎牛血清；1%雙抗
簡介	SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates.
形態(tài)	星狀細(xì)胞樣
生長特征	貼壁生長
倍增時(shí)間	每周 2 至 3 次
抗原表達(dá)	Blood Type O; Rh +; HLA A2, A11, B40, Bw16
致瘤性	Yes, in nude mice; forms malignant melanoma
STR	Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 THO1: 6,9 TPOX: 11 vWA: 14,18
培養(yǎng)條件	氣相：空氣，95% ；二氧化碳，5%。溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。
凍存條件	凍存液：90%FBS ，DMSO 10%, 或使用非程序凍存液：貨號JY-H040
保藏機(jī)構(gòu)	ATCC; HTB-70
產(chǎn)品使用	于科學(xué)研究，不可作為動物或人類疾病的治療產(chǎn)品使用。

19156044901 （同微信）

the mRNA levels of TGF- β1 and TIMP－2，and the protein levels of TGF- β1，TIMP－2，
and p- β-catenin / β-catenin were obviously increased (P＜0．05) ，the corresponding index of TAK－715 group showed the opposite trends ( P＜0．05) ;
compared with the ATR group，the axial length，refraction，the levels of IL－1β , IL－6 and TNF- α , the mRNA level of MMP－2，
and the protein lev- els of Wnt and MMP－2 in the ATR+TAK－715 group were obviously