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CNE人鼻咽癌細胞(附STR鑒

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更新時間:2025-02-11 18:13:11瀏覽次數:50次

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人鼻咽癌細胞CNE種屬人別稱CNE1; CNE疾病鼻咽癌傳代比例/細胞消化1:2傳代 ,消化2-3分鐘培養基配置RPMI1640培養基;10%胎牛血清;1%雙抗簡介此細胞株來源于一位58歲女性鼻咽癌患者

人鼻咽癌細胞CNE

種屬
別稱CNE1; CNE
疾病鼻咽癌
傳代比例/細胞消化1:2傳代 ,消化2-3分鐘
培養基配置RPMI1640培養基;10%胎牛血清;1%雙抗
簡介此細胞株來源于一位58歲女性鼻咽癌患者。Problematic cell line: Contaminated. On the basis of the STR
profile (PubMed =18196576) it is identical to CNE-2 and seems to be a hybrid of Hela and of cell line of unknow origin. This is also confirmed by RNASeq data from PubMed =24991015. Originally thought to originate from a 58 year old female patient with a nasopharyngeal carcinoma.
形態上皮細胞樣
生長特征貼壁生長
倍增時間每周 2 至 3
STRAmelogenin:X;CSF1PO:9 ,10;D13S317:13.3;D16S539:9 ,10;D18S51:16;D19S433:13 ,14;
D21S11:27 ,28;D2S1338:17;D3S1358:15 ,18;D5S818:11 ,12;D7S820:8 ,12;D8S1179:12 ,13;
FGA:18 ,21;TH01:7;TPOX:8 ,12;vWA:16 ,18
培養條件氣相:空氣 ,95% ;二氧化碳 ,5%。 溫度:37攝氏 ,培養箱濕度為70%-80%。
凍存條件凍存液:90%FBS ,DMSO 10%,
或使用非程序凍存液:貨號JY-H040
產品使用于科學研究 ,不可作為動物或人類疾病的治療產品使用。



Both resistance training (RT) and long-duration, high-intensity stretching induce muscular adaptations; however, it is unknown whether the modalities are complementary or redundant, particularly in well-trained individuals. A case-study was conducted on a competitive bodybuilder implementing long-duration, high-intensity stretching of the plantar flexors (60?min 6x/week for 12?weeks) in conjunction with their habitual RT. Ultrasound muscle architecture (muscle thickness [MT], fascicle length [FL], and pennation angle [PA]) measurements were collected at multiple sites at four weekly baseline sessions, six (mid) and 12 (post1) weeks following the commencement of the intervention, and a week after the intervention (post2) while isometric strength and range of motion (RoM) were obtained once at baseline, mid, post1, and post2. 2SD band plots were constructed to determine meaningful changes in MT, FL, and PA from the four baseline measures while percentage and absolute change across each timepoint were calculated for all variables. From baseline to post 1, RoM, strength, and MT increased 25.9%, 11.4%, and 7.4%-23.4%, respectively, while four MT and two PA sites exceeded the threshold for meaningful change. The combined stretching and RT protocols resulted in flexibility, strength, and MT adaptations; however, findings should be generalized with caution given the case-study nature of our investigation.


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