| CBP73184 | |
| I. Introduction | |
Cell Line Name： | Her2 A775_G776insYVMA/BaF3 |
Host Cell: | Ba/F3 |
| Stability: | 16 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
Application: | Anti-proliferation assay and PD assay |
Freeze Medium: | 90% FBS+10% DMSO |
Complete Culture Medium: | RPMI-1640+10%FBS |
Mycoplasma Status: | Negative |
| II.Background | |
ERBB2 (HER2) A775_G776insYVMA results in the insertion of four amino acids in the protein kinase domain of the Erbb2 (Her2) protein between amino acids 775 and 776 (UniProt.org). A775_G776insYVMA results in the same amino acid change as M774_A775insAYVM, which leads to constitutive phosphorylation of Erbb2 (Her2) and is transforming in cell culture (PMID: 17311002, PMID: 29967253). | |
| III. Representative Data | |
1. WB of Her2 A775_G776insYVMA/BaF3
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2. Anti-proliferation assay | |
Figure 2. Anti-proliferation assay of two reference compounds on the Her2 A775_G776insYVMA/BaF3 Stable Cell Line | |
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