| I. Background | |
| Programmed Cell Death Protein 1 (PD-1)，一種在激活的 T 細胞上表達的受體，與其配 體 PD-L1 和 PD-L2 結合，負向調節免疫反應。 PD-1 配體存在于大多數癌癥中，PD-1:PD- L1/2 相互作用會抑制 T 細胞活性，并使癌細胞逃避免疫監視。 PD1/PDL1 信號轉導通路是 腫瘤免疫抑制的重要組成部分，可以抑制 T 淋巴細胞的興奮，增強腫瘤細胞的免疫耐受， 從而實現腫瘤免疫逃逸。PD1 與 PDL1 結合可以減弱 T 細胞介導的免疫監視，導致免疫反 應缺失，甚至導致 T 細胞凋亡。PD1/PDL1 抑制劑可解除抗腫瘤 T 細胞的免疫抑制，從而 導致 T 細胞增殖并滲透到腫瘤微環境中并誘導抗腫瘤反應。PD-1:PD-L1/2 通路還參與調節 自身免疫反應，使這些蛋白質成為多種癌癥以及多發性硬化癥、關節炎、狼瘡和 I 型糖尿 病的有希望的治療靶點。磷酸酶 SHP2 是 T 細胞功能的關鍵調節因子，它介導 TCR 下游的激活信號以及 PD-1 下游的抑制信號。 | |
| II. Description | |
PDL1 TCR Activator CHO 細胞作為 PD1 NFAT-Luc Jurkat 細胞的靶細胞，很好的模擬了 體內 PD1&PDL1 的信號轉導過程，原理見下圖所示。
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| III. Introduction | |
| Host Cell: | CHO-K1 |
| Expressed gene: | PDL1、TCR Activator |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1 |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+2μg/ml puromycin+500μg/ml hygromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Cricetulus griseus, hamster, Chinese |
| Tissue: | Ovary |
| Disease: | Hamster Chinese ovary |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| Ⅴ. Representative Data | |
Figure 2.Recombinant PDL1/TCR Activator/CHO constitutively expressing PDL1.
Figure 3.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response of Anti-PD1 Neutralizing Antibody in PD1 NFAT-Luc Jurkat(C16) with PDL1 TCR Activator CHO(C5).
Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose response of Anti-PDL1 Neutralizing Antibody in PD1 NFAT-Luc Jurkat(C16) with PDL1 TCR Activator CHO(C5)..
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