| CBP74149 | |
| I. Background | |
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine stimulating the production of granulocytes and monocytes from bone marrow precursors. GM-CSF had pro-inflammatory functions and is a therapeutic target in autoimmune disease. GM-CSF signals through JAK2/STAT5 and stimulates the expression of STAT5 target genes. | |
| II. Introduction | |
| Expressed gene: | hGM-CSF |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 + 10% FBS +100ug/ml hygromycin+2 ng/ml GM-CSF |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Representative Data | |
Figure 1. Dose Response of Recombinant Human GM-CSF in hGM-CSF Effector Reporter Cells (Clone 10).
Figure 2. Inhibition of hGM-CSF-induced Reporter Activity by hGM-CSF Neutralizing Antibody in hGM-CSF Effector Reporter Cells (Clone10). | |
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